{"id":940,"date":"2019-06-07T16:40:24","date_gmt":"2019-06-07T14:40:24","guid":{"rendered":"http:\/\/www-pict.ipbs.fr\/?page_id=940"},"modified":"2022-12-16T14:50:44","modified_gmt":"2022-12-16T13:50:44","slug":"criblage","status":"publish","type":"page","link":"http:\/\/www.pict.ipbs.fr\/index.php\/criblage\/","title":{"rendered":"Criblage de Ligands"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"940\" class=\"elementor elementor-940\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3e2c84d2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3e2c84d2\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-4c42d268\" data-id=\"4c42d268\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-20c8973e elementor-widget elementor-widget-heading\" data-id=\"20c8973e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Identification de ligands innovants<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-49fa9295 elementor-widget elementor-widget-heading\" data-id=\"49fa9295\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Criblage de ligands par spectroscopie RMN, fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage (DSF) et cristallographie aux rayons X.<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9b83634 elementor-widget elementor-widget-text-editor\" data-id=\"9b83634\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: left;\"><span>La plateforme PICT dispose de plusieurs chimioth\u00e8ques (\u00ab drug-like \u00bb, peptide, fragments) et offre l\u2019acc\u00e8s et l\u2019expertise \u00e0 diff\u00e9rentes m\u00e9thodes compl\u00e9mentaires de criblage de ligands. <\/span><\/p><p style=\"text-align: left;\"><span>Ces m\u00e9thodes reposent sur l\u2019utilisation d\u2019\u00e9quipements et de m\u00e9thodes de biophysique de pointe, telles que la RMN, la\u00a0<\/span>cristallographie aux rayons X et la\u00a0fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage.<\/p><p style=\"text-align: left;\"><span>PICT vous propose ainsi de cribler ses chimioth\u00e8ques ou vos propres banques de mol\u00e9cules. Notre expertise en RMN pourra \u00eatre utilis\u00e9e en amont ou en aval du criblage pour valider et\/ou contr\u00f4ler vos chimioth\u00e8ques et d\u00e9terminer la solubilit\u00e9 des compos\u00e9s.<\/span><span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-002db89 elementor-widget elementor-widget-image\" data-id=\"002db89\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"300\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/complexe-ligand-proteine-300x300.jpg\" class=\"attachment-medium size-medium wp-image-1017\" alt=\"\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/complexe-ligand-proteine-300x300.jpg 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/complexe-ligand-proteine-150x150.jpg 150w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/complexe-ligand-proteine.jpg 480w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5fd143de elementor-widget elementor-widget-eael-adv-tabs\" data-id=\"5fd143de\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-adv-tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t        <div data-scroll-on-click=\"no\" data-scroll-speed=\"300\" id=\"eael-advance-tabs-5fd143de\" class=\"eael-advance-tabs eael-tabs-horizontal eael-tab-auto-active \" data-tabid=\"5fd143de\">\n            <div class=\"eael-tabs-nav \">\n                <ul class=\"\" role=\"tablist\">\n                                            <li id=\"criblage-par-rmn\" class=\"active-default eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"criblage-par-rmn-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Criblage par RMN<\/span>                                                    <\/li>\n                                            <li id=\"criblage-par-dsf\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"criblage-par-dsf-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Criblage par DSF<\/span>                                                    <\/li>\n                                            <li id=\"criblage-par-cristallographie-aux-rayons-x\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"criblage-par-cristallographie-aux-rayons-x-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Criblage par cristallographie aux rayons X<\/span>                                                    <\/li>\n                    \n                                  <\/ul>\n            <\/div>\n            \n            <div class=\"eael-tabs-content\">\n\t\t        \n                    <div id=\"criblage-par-rmn-tab\" class=\"clearfix eael-tab-content-item active-default\" data-title-link=\"criblage-par-rmn-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-48594584 elementor-widget elementor-widget-heading\"><div class=\"elementor-widget-container\"><h5 class=\"elementor-heading-title elementor-size-default\">Criblage par RMN<\/h5><\/div><\/div><section class=\"elementor-element elementor-element-12e7a384 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-2dd989c6 elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-6010b67 elementor-widget elementor-widget-heading\"><div class=\"elementor-widget-container\"><p class=\"elementor-heading-title elementor-size-default\">La spectroscopie RMN permet d\u2019identifier des interaction prot\u00e9ine-ligand et donc s\u2019inscrit comme une technique de choix dans le cadre d\u2019une campagne de criblage pour l\u2019identification de nouveaux ligands. La campagne de criblage s\u2019op\u00e8re selon deux \u00e9tapes successives :<\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-8f12346 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-0b71f55 elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-6a4b9df elementor-widget elementor-widget-image\"><div class=\"elementor-widget-container\"><div class=\"elementor-image\"><p><img decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/06\/FBS-300x119.jpg\" alt=\"\" class=\"size-medium wp-image-893\" width=\"300\" height=\"119\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/06\/FBS-300x119.jpg 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/06\/FBS-768x305.jpg 768w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/06\/FBS.jpg 900w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-b57d4bd elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-eb50682 elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-7714641 elementor-widget elementor-widget-text-editor\"><div class=\"elementor-widget-container\"><div class=\"elementor-text-editor elementor-clearfix\"><ul><li><strong>Campagne initiale de criblage pour l\u2019identification des ligands\u00a0(touches) :<\/strong> diff\u00e9rentes approches RMN tr\u00e8s robustes (telles que STD, WaterLOGSY \u2026) sont mises en \u0153uvre pour identifier l\u2019interaction de ligand d\u2019affinit\u00e9 (\u00b5M \u00e0 mM) avec une cible prot\u00e9ique, dans la cadre d\u2019une approche par fragment.<img decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/06\/CSP-300x164.jpg\" alt=\"\" class=\"size-medium wp-image-894 aligncenter\" width=\"300\" height=\"164\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/06\/CSP-300x164.jpg 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/06\/CSP.jpg 720w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/li><li><strong>\u00a0Identification du site d\u2019interaction :<\/strong> le site d\u2019interaction peut \u00eatre valid\u00e9 par une exp\u00e9rience de comp\u00e9tition (exp. STD, WaterLOGSY) avec un ligand connu ou en l\u2019absence de comp\u00e9titeur par une exp\u00e9rience de cartographie des d\u00e9placements chimiques de la prot\u00e9ine en pr\u00e9sence du ligand. Outre l\u2019identification du site d\u2019interaction, cette derni\u00e8re approche permet la construction d\u2019un mod\u00e8le d\u2019interaction.<\/li><\/ul><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-39add36 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-5098f0d elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-c07bfc7 elementor-widget elementor-widget-image\"><div class=\"elementor-widget-container\"><ul><\/ul><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-164720b9 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-1fc3d18a elementor-column elementor-col-50 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-6bb2323 elementor-align-center elementor-widget elementor-widget-button\"><div class=\"elementor-widget-container\"><div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-de-RMN-Biologique-Criblage-par-RMN.pdf\" class=\"elementor-button-link elementor-button elementor-size-sm\" target=\"_blank\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"elementor-element elementor-element-4e41bee5 elementor-column elementor-col-50 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-3200af77 elementor-align-center elementor-widget elementor-widget-button\"><div class=\"elementor-widget-container\"><div class=\"elementor-button-wrapper\"><a href=\"mailto:olivier.saurel@ipbs.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-address-book-o\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>                    <\/div>\n\t\t        \n                    <div id=\"criblage-par-dsf-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"criblage-par-dsf-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-7b68b22 elementor-widget elementor-widget-heading\"><div class=\"elementor-widget-container\"><div class=\"elementor-element elementor-element-b2a14aa elementor-widget elementor-widget-heading\"><div class=\"elementor-widget-container\"><div class=\"elementor-element elementor-element-48594584 elementor-widget elementor-widget-heading\"><div class=\"elementor-widget-container\"><div class=\"elementor-element elementor-element-b2a14aa elementor-widget elementor-widget-heading\"><div class=\"elementor-widget-container\"><div class=\"elementor-element elementor-element-5577886 elementor-widget elementor-widget-heading\"><div class=\"elementor-widget-container\"><h5 class=\"elementor-heading-title elementor-size-default\">Criblage par DSF<\/h5><\/div><\/div><div class=\"elementor-element elementor-element-6c640cb elementor-widget elementor-widget-text-editor\"><div class=\"elementor-widget-container\"><div class=\"elementor-text-editor elementor-clearfix\"><p>La\u00a0fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage\u00a0 (DSF) est une m\u00e9thode robuste afin d\u2019identifier des mol\u00e9cules susceptibles d\u2019interagir avec une prot\u00e9ine, soit pour l\u2019identification de partenaires physiologiques soit pour l\u2019identification de touches dans la cadre d\u2019un criblage de chimioth\u00e8ques.<\/p><\/div><\/div><\/div><section class=\"elementor-element elementor-element-350247a0 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-673cc46e elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-34e20c79 elementor-widget elementor-widget-image\"><div class=\"elementor-widget-container\"><div class=\"elementor-image\"><p><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/12\/DSF-300x225.png\" alt=\"\" class=\"size-medium wp-image-1119 aligncenter\" width=\"300\" height=\"225\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/12\/DSF-300x225.png 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/12\/DSF.png 447w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><div class=\"elementor-element elementor-element-60d6ebf elementor-widget elementor-widget-text-editor\"><div class=\"elementor-widget-container\"><div class=\"elementor-text-editor elementor-clearfix\"><p>Les prot\u00e9ines sont incub\u00e9es avec une sonde dont la fluorescence augmente lors d\u2019interactions avec les r\u00e9gions hydrophobes des prot\u00e9ines. Le d\u00e9pliement de la prot\u00e9ine, et l\u2019exposition des parties hydrophobes, donnent alors naissance \u00e0 un motif caract\u00e9ristique de la fluorescence en fonction de la temp\u00e9rature<strong>.<span>\u00a0 <\/span><\/strong><span>\u00a0<\/span><\/p><p>Les applications concernent non seulement le criblage de ligands mais \u00e9galement l\u2019identification de conditions optimales pour la purification et le stockage des prot\u00e9ines et de leurs partenaires pour faciliter la cristallisation. Du fait du peu de mat\u00e9riel prot\u00e9ique utilis\u00e9, du d\u00e9bit relativement \u00e9lev\u00e9 de la m\u00e9thode et de la large gamme d\u2019affinit\u00e9 couverte, la fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage est un outil pr\u00e9cieux et parfaitement adapt\u00e9 pour le criblage par fragment.<\/p><\/div><\/div><\/div><section class=\"elementor-element elementor-element-73645ef6 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-380b3e44 elementor-column elementor-col-50 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-16015e6c elementor-align-center elementor-widget elementor-widget-button\"><div class=\"elementor-widget-container\"><div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-de-Biophysique-criblage-DSF.pdf\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-sm\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-file-pdf\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"elementor-element elementor-element-6e509bf9 elementor-column elementor-col-50 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-4f567279 elementor-align-center elementor-widget elementor-widget-button\"><div class=\"elementor-widget-container\"><div class=\"elementor-button-wrapper\"><a href=\"mailto:virginie.nahoum@ipbs.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><\/div><\/div><section class=\"elementor-element elementor-element-f28c8de elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-a81f9a7 elementor-column elementor-col-50 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-126d814 elementor-align-center elementor-widget elementor-widget-button\"><div class=\"elementor-widget-container\"><div class=\"elementor-button-wrapper\"><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><\/div><\/div><\/div><\/div><\/div><\/div>                    <\/div>\n\t\t        \n                    <div id=\"criblage-par-cristallographie-aux-rayons-x-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"criblage-par-cristallographie-aux-rayons-x-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-48594584 elementor-widget elementor-widget-heading\"><div class=\"elementor-widget-container\"><h5 class=\"elementor-heading-title elementor-size-default\">Criblage par cristallographie aux rayons X<\/h5><\/div><\/div><section class=\"elementor-element elementor-element-12e7a384 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-2dd989c6 elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-05836f3 elementor-widget elementor-widget-text-editor\"><div class=\"elementor-widget-container\"><div class=\"elementor-text-editor elementor-clearfix\"><p>Lorsque les conditions de cristallisation d\u2019une macromol\u00e9cule sont connues, la d\u00e9termination de structures par cristallographie aux rayons X peut \u00eatre utilis\u00e9e pour cribler des chimioth\u00e8ques soit par co-cristallisation, soit par trempage.<\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-8f12346 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-0b71f55 elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-69d6235 elementor-widget elementor-widget-image\"><div class=\"elementor-widget-container\"><div class=\"elementor-image\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/12\/RX-300x199.png\" alt=\"\" class=\"size-medium wp-image-1120 aligncenter\" width=\"300\" height=\"199\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/12\/RX-300x199.png 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/12\/RX-360x240.png 360w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/12\/RX.png 383w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-b57d4bd elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-eb50682 elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-7714641 elementor-widget elementor-widget-text-editor\"><div class=\"elementor-widget-container\"><div class=\"elementor-text-editor elementor-clearfix\"><p>Les cristaux sont alors irradi\u00e9s par rayonnement synchrotron et les donn\u00e9es de diffraction collect\u00e9es. Les densit\u00e9s \u00e9lectroniques obtenues dans la structure des complexe macromol\u00e9cule\/ligand sont analys\u00e9es afin d\u2019identifier les complexes potentiels\u00a0macromol\u00e9cule\/ligand.<\/p><p>Les mol\u00e9cules interagissant de fa\u00e7on stable avec la macromol\u00e9cule peuvent alors \u00eatre identifi\u00e9es. Cette technique pr\u00e9sente l\u2019avantage d\u2019identifier des mol\u00e9cules interagissant de fa\u00e7on stable avec la prot\u00e9ine mais \u00e9galement de pr\u00e9ciser les d\u00e9tails des interactions au niveau atomique.<\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-39add36 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-5098f0d elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap\"><div class=\"elementor-widget-wrap\"><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-8e603cc elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-d7d755d elementor-column elementor-col-100 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-06a1ae6 elementor-widget elementor-widget-text-editor\"><div class=\"elementor-widget-container\"><div class=\"elementor-text-editor elementor-clearfix\"><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"elementor-element elementor-element-164720b9 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\"><div class=\"elementor-container elementor-column-gap-default\"><div class=\"elementor-row\"><div class=\"elementor-element elementor-element-1fc3d18a elementor-column elementor-col-50 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-6bb2323 elementor-align-center elementor-widget elementor-widget-button\"><div class=\"elementor-widget-container\"><div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-de-Bio-Cristallographie-criblage.pdf\" class=\"elementor-button-link elementor-button elementor-size-sm\" target=\"_blank\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-file-pdf\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"elementor-element elementor-element-4e41bee5 elementor-column elementor-col-50 elementor-inner-column\"><div class=\"elementor-column-wrap elementor-element-populated\"><div class=\"elementor-widget-wrap\"><div class=\"elementor-element elementor-element-3200af77 elementor-align-center elementor-widget elementor-widget-button\"><div class=\"elementor-widget-container\"><div class=\"elementor-button-wrapper\"><a href=\"mailto:virginie.nahoum@ipbs.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/section>                    <\/div>\n\t\t                    <\/div>\n        <\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2ff849d3 elementor-widget elementor-widget-heading\" data-id=\"2ff849d3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Offre de service<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4bf748cb elementor-widget elementor-widget-text-editor\" data-id=\"4bf748cb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span>Notre offre de service en biologie structurale couvre l\u2019ensemble de nos expertises. Elle peut prendre differentes formes allant de la <\/span><strong>prestation de service \u00e0 la collaboration scientifique. <\/strong><span>Une <\/span><strong>\u00e9tude de faisabilit\u00e9<\/strong><span> est souvent n\u00e9cessaire.\u00a0<\/span><\/p><p>La \u00a0DSF (fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage) est une m\u00e9thode tr\u00e8s rapide permettant de cribler jusqu\u2019\u00e0 1000 compos\u00e9s en 2 jours sur la base d\u2019une interaction directe entre la cible et le ligand.<\/p><p>La spectroscopie RMN permet de cribler rapidement (en moins d\u2019une semaine), une librairie de taille \u00e9quivalente. <span>\u00a0<\/span>Il est en plus possible de valider le site de fixation du ligand en utilisant par exemple un comp\u00e9titeur d\u00e9j\u00e0 connu.<\/p><p>Lorsque les conditions de cristallisation de la cible sont connues, la cristallographie aux rayons X peut \u00eatre utilis\u00e9e comme m\u00e9thode de criblage. Elle est moins rapide que la RMN mais pr\u00e9sente l\u2019avantage de fournir en m\u00eame temps une identification du site d\u2019interaction et une compr\u00e9hension du mode d\u2019interaction.<\/p><p><strong>Une \u00e9tude ambitieuse de d\u00e9couverte de ligands fait g\u00e9n\u00e9ralement appel aux trois approches selon une strat\u00e9gie en trois \u00e9tapes successives: criblage initial (ex. DSF et RMN), confirmation des touches (RMN) et caract\u00e9risation structurale du complexe ligand-prot\u00e9ine (cristallographie aux rayons X\u00a0ou RMN).<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-37b3f03b elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"37b3f03b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-19153e77 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"19153e77\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Identification de ligands innovants Criblage de ligands par spectroscopie RMN, fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage (DSF) et cristallographie aux rayons X. La plateforme PICT dispose de plusieurs chimioth\u00e8ques (\u00ab drug-like \u00bb, peptide, fragments) et offre l\u2019acc\u00e8s et l\u2019expertise \u00e0 diff\u00e9rentes m\u00e9thodes compl\u00e9mentaires de criblage de ligands. Ces m\u00e9thodes reposent sur l\u2019utilisation [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":230,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/template-fullwidth.php","meta":{"footnotes":""},"class_list":["post-940","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/940","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/comments?post=940"}],"version-history":[{"count":85,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/940\/revisions"}],"predecessor-version":[{"id":1896,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/940\/revisions\/1896"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/media\/230"}],"wp:attachment":[{"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/media?parent=940"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}