{"id":964,"date":"2019-06-07T16:50:03","date_gmt":"2019-06-07T14:50:03","guid":{"rendered":"http:\/\/www-pict.ipbs.fr\/?page_id=964"},"modified":"2023-01-30T13:34:00","modified_gmt":"2023-01-30T12:34:00","slug":"biophysique","status":"publish","type":"page","link":"http:\/\/www.pict.ipbs.fr\/index.php\/biophysique\/","title":{"rendered":"Etude des macromol\u00e9cules et leurs complexes"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"964\" class=\"elementor elementor-964\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-24b5549f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"24b5549f\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-57c3a056\" data-id=\"57c3a056\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-66424e9d elementor-widget elementor-widget-text-editor\" data-id=\"66424e9d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\n<div class=\"wp-block-themeisle-blocks-advanced-columns has-undefined-columns has-desktop-undefined-layout has-tablet-equal-layout has-mobile-equal-layout has-default-gap has-vertical-unset\" id=\"wp-block-themeisle-blocks-advanced-columns-fa3705ef\" style=\"border-width:0px;border-style:solid;border-color:#000000;border-radius:0px;justify-content:unset\"><div class=\"wp-themeisle-block-overlay\" style=\"opacity:0.5;mix-blend-mode:normal\"><\/div><div class=\"innerblocks-wrap\"><\/div><\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-68fcec3d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"68fcec3d\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-a08bfc7\" data-id=\"a08bfc7\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7d428165 elementor-widget elementor-widget-heading\" data-id=\"7d428165\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Caract\u00e9risation biophysique des macromol\u00e9cules et leurs complexes : <\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-12533176 elementor-widget elementor-widget-heading\" data-id=\"12533176\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">monodispersit\u00e9, oligom\u00e9risation, masse mol\u00e9culaire des prot\u00e9ines et leurs complexes, mesure thermodynamique des interactions.<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-200fe58d elementor-widget elementor-widget-text-editor\" data-id=\"200fe58d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Le plateau de Biophysique dispose de l\u2019instrumentation et l\u2019expertise pour la caract\u00e9risation des macromol\u00e9cules et des interactions mol\u00e9culaires en faisant appel \u00e0 plusieurs m\u00e9thodes biophysiques, incluant la <strong>diffusion de lumi\u00e8re statique et dynamique (DLS, SLS)<\/strong>, le <strong>dichro\u00efsme circulaire (CD)<\/strong>, la\u00a0<strong>fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage\u00a0(DSF)<\/strong> et <strong>la titration calorim\u00e9trique isotherme (ITC)<\/strong>. Ces m\u00e9thodes permettent notamment de mesurer la solubilit\u00e9, la stabilit\u00e9, la masse molaire ainsi que l\u2019\u00e9tat de polydispersit\u00e9 et d\u2019oligom\u00e9risation des macromol\u00e9cules et leurs complexes.<\/p>\n<p>Ces \u00e9quipements peuvent notamment \u00eatre utilis\u00e9s en amont d\u2019une <strong>\u00e9tude structurale<\/strong> ou d\u2019un <strong>criblage<\/strong> pour caract\u00e9riser les \u00e9chantillons prot\u00e9iques et v\u00e9rifier qu\u2019ils remplissent les conditions n\u00e9cessaires \u00e0 l\u2019\u00e9tude envisag\u00e9e.<\/p>\n<p><\/p>\n<p>Elles sont \u00e9galement utilis\u00e9es pour caract\u00e9riser en d\u00e9tail le <strong>mode d\u2019interaction<\/strong> de diff\u00e9rents types <strong>de<\/strong> <strong>complexes macromol\u00e9culaires (interaction prot\u00e9ine-prot\u00e9ine (PPI), ligands, acides nucl\u00e9iques)<\/strong> et suivre les changements conformationnels pouvant en r\u00e9sulter.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-750b7402 elementor-widget elementor-widget-heading\" data-id=\"750b7402\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Pr\u00e9requis<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-51653ee5 elementor-widget elementor-widget-text-editor\" data-id=\"51653ee5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Toute \u00e9tude biophysique requiert un \u00e9chantillon prot\u00e9ique pur chimiquement. Cette puret\u00e9 doit \u00eatre valid\u00e9e \u00e0 minima par analyse sur gel en conditions d\u00e9naturantes. Certaines m\u00e9thodes, comme le microcalorim\u00e9trie demandent en plus une puret\u00e9 oligom\u00e9rique.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-69809602 elementor-widget elementor-widget-heading\" data-id=\"69809602\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Offre de service<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-51f763b8 elementor-widget elementor-widget-eael-adv-tabs\" data-id=\"51f763b8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-adv-tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t        <div data-scroll-on-click=\"no\" data-scroll-speed=\"300\" id=\"eael-advance-tabs-51f763b8\" class=\"eael-advance-tabs eael-tabs-horizontal eael-tab-auto-active \" data-tabid=\"51f763b8\">\n            <div class=\"eael-tabs-nav \">\n                <ul class=\"\" role=\"tablist\">\n                                            <li id=\"dsf\" class=\"active-default eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"dsf-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >DSF<\/span>                                                    <\/li>\n                                            <li id=\"dls-sec-mals\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"dls-sec-mals-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >DLS &amp; SEC-MALS<\/span>                                                    <\/li>\n                                            <li id=\"itc\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"itc-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >ITC<\/span>                                                    <\/li>\n                                            <li id=\"dichrosme-circulaire\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"4\" role=\"tab\" tabindex=\"-1\" aria-controls=\"dichrosme-circulaire-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Dichro\u00efsme Circulaire<\/span>                                                    <\/li>\n                    \n                                  <\/ul>\n            <\/div>\n            \n            <div class=\"eael-tabs-content\">\n\t\t        \n                    <div id=\"dsf-tab\" class=\"clearfix eael-tab-content-item active-default\" data-title-link=\"dsf-tab\">\n\t\t\t\t        <section class=\"elementor-element elementor-element-2b3ac26 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-top-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-24990ea elementor-column elementor-col-100 elementor-top-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-38232d4 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-element elementor-element-43240e34 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<h5 class=\"elementor-heading-title elementor-size-default\">Fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage (DSF)<\/h5>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-4497e4b elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p>C\u2019est une m\u00e9thode simple et rapide utilis\u00e9e en format 96 (96 conditions test\u00e9es simultan\u00e9ment) pour cribler diff\u00e9rents tampons, pH, salinit\u00e9, cofacteurs, acides nucl\u00e9iques, \u2026<\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-3b70451 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-3d831aa2 elementor-column elementor-col-100 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-73ae46f8 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img fetchpriority=\"high\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/09\/DSF-2-300x300.jpg\" alt=\"\" class=\"alignnone size-medium wp-image-1109\" width=\"300\" height=\"300\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/09\/DSF-2-300x300.jpg 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/09\/DSF-2-150x150.jpg 150w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/09\/DSF-2.jpg 500w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<div class=\"elementor-element elementor-element-22a2600 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/DSF-plot-300x180.jpg\" alt=\"\" class=\"alignnone size-medium wp-image-1141\" width=\"300\" height=\"180\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/DSF-plot-300x180.jpg 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/DSF-plot.jpg 313w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-a90caf7 elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p>Son format haut d\u00e9bit est parfaitement adapt\u00e9 au criblage de petites chimioth\u00e8ques de type fragments.<\/p>\n<p>Dans cette m\u00e9thode de criblage on mesure des changements dans la stabilit\u00e9 thermique de la prot\u00e9ine selon qu\u2019elle soit seule ou en complexe avec un ligand.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-4f25540d elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-6e2bc895 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-8182bda elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-de-Biophysique-DSF.pdf\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-sm\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-708bc148 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3ac423fd elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"mailto:virginie.nahoum@ipbs.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-2268bbf elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-23e2039 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-78d1fad elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>                    <\/div>\n\t\t        \n                    <div id=\"dls-sec-mals-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"dls-sec-mals-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-7b68b22 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-element elementor-element-43240e34 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<h5 class=\"elementor-heading-title elementor-size-default\">DLS &amp; SEC-MALS<\/h5>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-4497e4b elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p>La diffusion dynamique de la lumi\u00e8re (DLS) permet de caract\u00e9riser l\u2019homog\u00e9n\u00e9it\u00e9 des solutions (mesure de la polydispersit\u00e9), le taux d\u2019agr\u00e9gation et la taille des particules (prot\u00e9ines, ADN-ARN, complexes)\u00a0par l\u2019estimation du rayon hydrodynamique (Rh) des populations pr\u00e9sentes.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-3b70451 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-3d831aa2 elementor-column elementor-col-100 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-73ae46f8 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/secmals-10.jpg\" alt=\"\" class=\"alignnone size-full wp-image-1146\" width=\"286\" height=\"167\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<div class=\"elementor-element elementor-element-12efafe9 elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p>La chromatographie d\u2019exclusion (SEC) coupl\u00e9e \u00ab en ligne \u00bb avec les mesures de la diffusion statique de la lumi\u00e8re \u00e0 plusieurs angles (MALS), de l\u2019index de r\u00e9fraction (RI) et de l\u2019absorbance (UV) est une m\u00e9thode rapide et pr\u00e9cise pour d\u00e9terminer la masse molaire des prot\u00e9ines et des complexes en solution et l\u2019\u00e9tat d\u2019oligom\u00e9rie. Elle permet \u00e9galement de contr\u00f4ler l\u2019homog\u00e9n\u00e9it\u00e9 conformationnelle des \u00e9chantillons.<\/p>\n<div class=\"elementor-element elementor-element-38232d4 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-element elementor-element-22a2600 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/SEC-MALS-1-300x239.jpg\" alt=\"\" class=\"alignnone wp-image-1091 size-medium\" width=\"300\" height=\"239\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/SEC-MALS-1-300x239.jpg 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/SEC-MALS-1.jpg 501w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-4f25540d elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-6e2bc895 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-8182bda elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-de-Biophysique-DSF.pdf\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-sm\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-708bc148 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3ac423fd elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"mailto:virginie.nahoum@ipbs.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-2268bbf elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-23e2039 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-78d1fad elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>                    <\/div>\n\t\t        \n                    <div id=\"itc-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"itc-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-43240e34 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<h5 class=\"elementor-heading-title elementor-size-default\">L\u2019ITC (titrage calorim\u00e9trique isotherme)<\/h5>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-4497e4b elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p>L\u2019ITC ou (titrage calorim\u00e9trique isotherme) permet de <span>mesurer la <\/span><a href=\"https:\/\/fr.wikipedia.org\/wiki\/Chaleur_de_r%C3%A9action\"><span>chaleur de r\u00e9action<\/span><\/a><span> d\u2019une solution en fonction du volume du <\/span><a href=\"https:\/\/fr.wikipedia.org\/wiki\/R%C3%A9actif_(chimie)\"><span>r\u00e9actif<\/span><\/a> <a href=\"https:\/\/fr.wikipedia.org\/w\/index.php?title=Titrant&amp;action=edit&amp;redlink=1\"><span>titrant<\/span><\/a><span> ajout\u00e9, et<\/span> de d\u00e9terminer les param\u00e8tres thermodynamiques associ\u00e9s \u00e0 l\u2019interaction entre mol\u00e9cules de tout type (prot\u00e9ines, ADN, petites mol\u00e9cules\u2026).<\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-3b70451 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-3d831aa2 elementor-column elementor-col-100 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-73ae46f8 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/ITC-1-199x300.jpg\" alt=\"\" class=\"alignnone size-medium wp-image-1094\" width=\"199\" height=\"300\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/ITC-1-199x300.jpg 199w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2019\/07\/ITC-1.jpg 265w\" sizes=\"(max-width: 199px) 100vw, 199px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<div class=\"elementor-element elementor-element-a90caf7 elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p>Le plateau de biophysique est \u00e9quip\u00e9 d\u2019un calorim\u00e8tre isotherme ITC200 enti\u00e8rement d\u00e9di\u00e9 \u00e0 l\u2019\u00e9tude des interactions mol\u00e9culaires. Cet appareil permet de mesurer une constante d\u2019association en solution et ceci sans n\u00e9cessit\u00e9 de marquer les mol\u00e9cules. La gamme d\u2019affinit\u00e9s nM-mM peut \u00eatre mesur\u00e9e de mani\u00e8re directe et des affinit\u00e9s de l\u2019ordre du picomolaire peuvent \u00eatre estim\u00e9es gr\u00e2ce \u00e0 des exp\u00e9riences de comp\u00e9tition.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-4f25540d elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-6e2bc895 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-8182bda elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-708bc148 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3ac423fd elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"mailto:valerie.guillet@ipbs.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>                    <\/div>\n\t\t        \n                    <div id=\"dichrosme-circulaire-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"dichrosme-circulaire-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-43240e34 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-element elementor-element-43240e34 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<h5 class=\"elementor-heading-title elementor-size-default\">Dichro\u00efsme Circulaire (CD)<\/h5>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-4497e4b elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><span>Dans le cas des prot\u00e9ines et des peptides, le dichro\u00efsme circulaire permet d\u2019\u00e9valuer le niveau de structuration de la macromol\u00e9cule (repliement) et d\u2019estimer les pourcentages de structures secondaires (h\u00e9lices alpha, feuillet beta, random coil). La technique n\u2019\u00e9tant pas destructive il est possible d\u2019effectuer des cin\u00e9tiques ou titrations pour suivre les changements conformationnels. Il est \u00e9galement possible de d\u00e9terminer la temp\u00e9rature de fusion (Tm) de la macromol\u00e9cule et ceci sans la pr\u00e9sence d\u2019un fluorophore, ce qui rend la m\u00e9thode CD compl\u00e9mentaire \u00e0 la DSF. D\u2019autres types de mol\u00e9cules sont analysables comme les acides nucl\u00e9iques (ADN, ARN), les sucres et \u00e9galement les petites mol\u00e9cules.<\/span><span><\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-9fc3eaf elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/Jasco-J-815.png\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/Jasco-J-815.png\" class=\"attachment-medium alignnone\" alt=\"\" srcset=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/Jasco-J-815.png 300w, http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/Jasco-J-815.png 464w\" sizes=\"(max-width: 300px) 100vw, 300px\" width=\"300\" height=\"226\" \/><\/a><\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-12efafe9 elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><strong><em>Pr\u00e9requis\u00a0:<\/em><\/strong><\/p>\n<p>Macromol\u00e9cule pure \u00e0 \u2265 90%. La quantit\u00e9 et concentration d\u00e9pendent essentiellement de la cuvette utilis\u00e9e : de quelques \u00b5g\/ml pour la cuvette 500\u00b5L (chemin optique 1mm) \u00e0 quelques mg\/ml pour la cuvette sandwich (chemin optique 0.01mm). Les meilleurs tampons sont le phosphate, le borate et le Tris-HCl. Eviter des fortes concentrations en NaCl et toutes mol\u00e9cules qui absorbent<span>\u00a0 <\/span>fortement dans l\u2019UV.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-4f25540d elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-6e2bc895 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-8182bda elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-ICEO-Dichroisme-Circulaire.pdf\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-sm\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-708bc148 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3ac423fd elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"mailto:iceo@insa-toulouse.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-4f25540d elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-708bc148 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3ac423fd elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>                    <\/div>\n\t\t                    <\/div>\n        <\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-17ca7bfc elementor-widget elementor-widget-text-editor\" data-id=\"17ca7bfc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>L&rsquo;ensemble de ces m\u00e9thodes entre dans le cadre des prestations pour caract\u00e9riser les macromol\u00e9cules et leurs complexes. Selon vos besoins ou la nature du sujet, l&rsquo;offre de service est large : \u00a0mise \u00e0 disposition des \u00e9quipements (apr\u00e8s une formation pr\u00e9alable), contrat de prestation de service ou collaboration scientifique.<\/p>\n<ul>\n<li>La<strong> DSF<\/strong> (fluorim\u00e9trie diff\u00e9rentielle \u00e0 balayage) est utilis\u00e9e pour la d\u00e9termination de la temp\u00e9rature de d\u00e9naturation d\u2019une prot\u00e9ine. Cette m\u00e9thode permet de cribler tr\u00e8s rapidement diff\u00e9rents conditionnements de prot\u00e9ine (pH, tampon, sel, \u2026), ou d\u2019\u00e9tudier la formation de complexes (prot\u00e9ine\/ligand, prot\u00e9ine\/acide nucl\u00e9ique).<\/li>\n<li>La <strong>DLS<\/strong> et le <strong>SEC-MALS<\/strong> permettent de mesurer l\u2019\u00e9tat d\u2019oligom\u00e9risation et de d\u00e9terminer la masse molaire de l\u2019\u00e9chantillon prot\u00e9ique.<\/li>\n<li>L\u2019<strong>ITC<\/strong> permet de d\u00e9terminer l\u2019affinit\u00e9 et les bases thermodynamiques des interactions mol\u00e9culaires.<\/li>\n<li>Le <strong>dichro\u00efsme circulaire (CD)<\/strong> permet d\u2019\u00e9valuer le repliement prot\u00e9ique et les structures secondaires d\u2019un \u00e9chantillon prot\u00e9ique.<\/li>\n<\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5a936e4a elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"5a936e4a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-67dab9f2 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"67dab9f2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Caract\u00e9risation biophysique des macromol\u00e9cules et leurs complexes : monodispersit\u00e9, oligom\u00e9risation, masse mol\u00e9culaire des prot\u00e9ines et leurs complexes, mesure thermodynamique des interactions. Le plateau de Biophysique dispose de l\u2019instrumentation et l\u2019expertise pour la caract\u00e9risation des macromol\u00e9cules et des interactions mol\u00e9culaires en faisant appel \u00e0 plusieurs m\u00e9thodes biophysiques, incluant la diffusion de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":230,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/template-fullwidth.php","meta":{"footnotes":""},"class_list":["post-964","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/964","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/comments?post=964"}],"version-history":[{"count":61,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/964\/revisions"}],"predecessor-version":[{"id":1938,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/964\/revisions\/1938"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/media\/230"}],"wp:attachment":[{"href":"http:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/media?parent=964"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}