{"id":954,"date":"2019-06-07T16:46:34","date_gmt":"2019-06-07T14:46:34","guid":{"rendered":"http:\/\/www-pict.ipbs.fr\/?page_id=954"},"modified":"2022-12-16T15:25:56","modified_gmt":"2022-12-16T14:25:56","slug":"iceo","status":"publish","type":"page","link":"https:\/\/www.pict.ipbs.fr\/index.php\/iceo\/","title":{"rendered":"Criblage d&rsquo;enzymes"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"954\" class=\"elementor elementor-954\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-103b2f52 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"103b2f52\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-4632c3ef\" data-id=\"4632c3ef\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b6f83b7 elementor-widget elementor-widget-text-editor\" data-id=\"b6f83b7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\n<div class=\"wp-block-themeisle-blocks-advanced-columns has-undefined-columns has-desktop-undefined-layout has-tablet-equal-layout has-mobile-equal-layout has-default-gap has-vertical-unset\" id=\"wp-block-themeisle-blocks-advanced-columns-b83c6937\" style=\"border-width:0px;border-style:solid;border-color:#000000;border-radius:0px;justify-content:unset\"><div class=\"wp-themeisle-block-overlay\" style=\"opacity:0.5;mix-blend-mode:normal\"><\/div><div class=\"innerblocks-wrap\"><\/div><\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-454ec06c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"454ec06c\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-19e98757\" data-id=\"19e98757\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2fccbc33 elementor-widget elementor-widget-heading\" data-id=\"2fccbc33\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Ing\u00e9nierie et d\u00e9couverte d\u2019enzymes originales\n<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-366b57ee elementor-widget elementor-widget-heading\" data-id=\"366b57ee\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Ing\u00e9nierie rationnelle et \u00e9volution dirig\u00e9e d\u2019enzymes; d\u00e9couverte d\u2019enzymes par m\u00e9tag\u00e9nomique fonctionnelle; criblage haut-d\u00e9bit d\u2019activit\u00e9s enzymatiques; production et purification d\u2019enzymes\n<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-12d2c084 elementor-widget elementor-widget-text-editor\" data-id=\"12d2c084\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span>Le plateau PICT-ICEO <\/span><span>est d\u00e9di\u00e9 au criblage et \u00e0 l\u2019\u00e9tude d\u2019activit\u00e9s enzymatiques originales, isol\u00e9es \u00e0 partir de librairies g\u00e9n\u00e9r\u00e9es par ing\u00e9nierie combinatoire des prot\u00e9ines ou issues de <\/span><span>collections, <\/span><span>de banques g\u00e9nomiques ou <\/span><span>m\u00e9tag\u00e9nomiques<\/span><span>. <\/span><\/p>\n<p><span>Notre expertise et nos services sont ax\u00e9s autour : <\/span><\/p>\n<p><span>&#8211; de l\u2019exploration \u00e0 haut-d\u00e9bit de la diversit\u00e9 fonctionnelle enzymatique,<\/span><\/p>\n<p><span>&#8211; de la compr\u00e9hension des relations <\/span><span>s\u00e9quence-structure-fonction <\/span><span>d\u2019enzymes <\/span><span>cibles. <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-68f7980 elementor-widget elementor-widget-heading\" data-id=\"68f7980\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Offre de service<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-42776bdc elementor-widget elementor-widget-text-editor\" data-id=\"42776bdc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span>Le plateau PICT-ICEO propose des services qui vont de la cr\u00e9ation de banques g\u00e9nomiques ou m\u00e9tag\u00e9nomique, \u00e0 la g\u00e9n\u00e9ration de diversit\u00e9 mol\u00e9culaire (avec m\u00e9thode al\u00e9atoire, semi-rationnelle ou rationnelle), le criblage d\u2019activit\u00e9s enzymatiques et la production d\u2019enzymes \u00e0 plus large \u00e9chelle. Cette offre <\/span><span>peut <\/span><span>prendre <\/span><span>diff\u00e9rentes <\/span><span>formes allant de la <\/span><span>prestation de service \u00e0 la collaboration <\/span><span>scientifique, en passant par la mise \u00e0 disposition d\u2019\u00e9quipements apr\u00e8s formation. <\/span><span>Nous vous accompagnons dans vos projets, de la conception des m\u00e9thodes de criblage adapt\u00e9es au choix des diversit\u00e9s \u00e0 explorer, jusqu\u2019\u00e0 la production des variantes pour la caract\u00e9risation\u00a0 fonctionnelle et structurale.\u00a0\u00a0\u00a0 <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2d7f7467 elementor-widget elementor-widget-eael-adv-tabs\" data-id=\"2d7f7467\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-adv-tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t        <div data-scroll-on-click=\"no\" data-scroll-speed=\"300\" id=\"eael-advance-tabs-2d7f7467\" class=\"eael-advance-tabs eael-tabs-horizontal eael-tab-auto-active \" data-tabid=\"2d7f7467\">\n            <div class=\"eael-tabs-nav \">\n                <ul class=\"\" role=\"tablist\">\n                                            <li id=\"banque-de-clones\" class=\"active-default eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"banque-de-clones-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Banque de clones<\/span>                                                    <\/li>\n                                            <li id=\"evolution-dirige\" class=\" eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"evolution-dirige-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Evolution dirig\u00e9e<\/span>                                                    <\/li>\n                                            <li id=\"criblage-denzymes\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"criblage-denzymes-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Criblage d'enzymes<\/span>                                                    <\/li>\n                                            <li id=\"purification-denzymes\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"4\" role=\"tab\" tabindex=\"-1\" aria-controls=\"purification-denzymes-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Purification d'enzymes<\/span>                                                    <\/li>\n                    \n                                  <\/ul>\n            <\/div>\n            \n            <div class=\"eael-tabs-content\">\n\t\t        \n                    <div id=\"banque-de-clones-tab\" class=\"clearfix eael-tab-content-item active-default\" data-title-link=\"banque-de-clones-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-4b668149 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<h5 class=\"elementor-heading-title elementor-size-default\">Picking et organisation de banque de clones<\/h5>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-110e6bf elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><span>L\u2019exploration <\/span><span>de la diversit\u00e9 issue de l\u2019ing\u00e9nierie <\/span><span>combinatoire, des <\/span><span>g\u00e9nomes et des <\/span><span>m\u00e9tag\u00e9nomes<\/span><span>, <\/span><span>exige de <\/span><span>g\u00e9n\u00e9rer et de cribler des banques pour isoler <\/span><span>les clones <\/span><span>les plus int\u00e9ressants<\/span><span>. Afin <\/span><span>de pouvoir \u00eatre \u00e9tudi\u00e9 individuellement, <\/span><span>chaque mutant <\/span><span>doit \u00eatre organis\u00e9 en microplaques, afin <\/span><span>de permettre <\/span><span>la robotisation de son analyse <\/span><span>ult\u00e9rieure ainsi <\/span><span>que sa conservation \u00e0 long terme.<\/span><span>\u00a0\u00a0<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-448bb046 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-52690d63 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3a83c8b3 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img fetchpriority=\"high\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/picking-clones-1-300x225.png\" alt=\"\" class=\"alignnone size-medium wp-image-1174\" width=\"300\" height=\"225\" srcset=\"https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/picking-clones-1-300x225.png 300w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/picking-clones-1.png 416w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-bd4e33 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-332bba2d elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/picking-clones-2-300x250.png\" alt=\"\" class=\"alignnone size-medium wp-image-1176\" width=\"300\" height=\"250\" srcset=\"https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/picking-clones-2-300x250.png 300w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/picking-clones-2.png 375w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<div class=\"elementor-element elementor-element-61eed129 elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p>Cette prestation se propose d\u2019organiser en milieu liquide au format microplaques 96 ou 384 puits:<\/p>\n<p>&#8211; des librairies de variants issues des techniques de mutag\u00e9n\u00e8se dirig\u00e9e, semi-al\u00e9atoire et\/ou al\u00e9atoire<\/p>\n<p>&#8211; des banques g\u00e9nomiques ou m\u00e9tag\u00e9nomiques (g\u00e9n\u00e9r\u00e9es \u00e0 partir de l\u2019ADN g\u00e9nomique de bact\u00e9ries non cultivables).<\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-50205293 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-39f93019 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-453a6df5 elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-ICEO-Banque-de-Clones.pdf\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-sm\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-a5346bc elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-1054e2ff elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"mailto:iceo@insa-toulouse.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>                    <\/div>\n\t\t        \n                    <div id=\"evolution-dirige-tab\" class=\"clearfix eael-tab-content-item \" data-title-link=\"evolution-dirige-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-4b668149 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<h5 class=\"elementor-heading-title elementor-size-default\">Evolution dirig\u00e9e<\/h5>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-110e6bf elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><span>Les techniques d\u2019ing\u00e9nierie des prot\u00e9ines actuelles permettent d\u2019optimiser quasiment \u00e0 fa\u00e7on les biocatalyseurs d\u2019int\u00e9r\u00eat. Que ce soit pour augmenter une activit\u00e9 catalytique, am\u00e9liorer une r\u00e9sistance physico-chimique, ou modifier une sp\u00e9cificit\u00e9 de r\u00e9actions, le principe de l\u2018\u00e9volution dirig\u00e9e des prot\u00e9ines permet d\u2019\u00e9largir consid\u00e9rablement les perspectives de mise en \u0153uvre d\u2019une enzyme dans un proc\u00e9d\u00e9 biotechnologique robuste ou dans une voie m\u00e9tabolique innovante.<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-448bb046 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-52690d63 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3a83c8b3 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/shufling-1-300x190.png\" alt=\"\" class=\"alignnone size-medium wp-image-1180\" width=\"300\" height=\"190\" srcset=\"https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/shufling-1-300x190.png 300w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/shufling-1.png 527w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-bd4e33 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-332bba2d elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/shuffling-2-300x224.png\" alt=\"\" class=\"alignnone size-medium wp-image-1181\" width=\"300\" height=\"224\" srcset=\"https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/shuffling-2-300x224.png 300w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/shuffling-2.png 456w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<div class=\"elementor-element elementor-element-61eed129 elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><span>La <\/span><span>prestation de Cr\u00e9ation de Diversit\u00e9 offre au client la possibilit\u00e9 de :<\/span><\/p>\n<p><span>\u2013\u00a0 mettre au point une strat\u00e9gie de g\u00e9n\u00e9ration de diversit\u00e9 g\u00e9nique pour une prot\u00e9ine d\u2019int\u00e9r\u00eat<\/span><\/p>\n<p><span>\u2013\u00a0 construire une banque de mutants de ce g\u00e8ne d\u2019int\u00e9r\u00eat<\/span><\/p>\n<p><span>\u2013\u00a0 organiser cette banque au format microplaque.<\/span><span><\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-50205293 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-39f93019 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-453a6df5 elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-ICEO-Evolution-dirigCe.pdf\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-sm\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-a5346bc elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-1054e2ff elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"mailto:iceo@insa-toulouse.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>                    <\/div>\n\t\t        \n                    <div id=\"criblage-denzymes-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"criblage-denzymes-tab\">\n\t\t\t\t        <section class=\"elementor-element elementor-element-3a2002ef elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-top-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-50af480e elementor-column elementor-col-100 elementor-top-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-4b668149 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<h5 class=\"elementor-heading-title elementor-size-default\">Criblage<\/h5>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-110e6bf elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><span>La r\u00e9ussite de tout projet d\u2019\u00e9volution dirig\u00e9e repose sur la mise au point attentive d\u2019un protocole de s\u00e9lection ou de criblage permettant de discriminer avec robustesse et efficacit\u00e9 les mutants les plus int\u00e9ressants au regard de la propri\u00e9t\u00e9 consid\u00e9r\u00e9e. <\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-448bb046 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-52690d63 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3a83c8b3 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/criblage-enz-1-300x196.png\" alt=\"\" class=\"alignnone size-medium wp-image-1184\" width=\"300\" height=\"196\" srcset=\"https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/criblage-enz-1-300x196.png 300w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/criblage-enz-1.png 424w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-bd4e33 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-332bba2d elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/criblage-enz-2-300x201.png\" alt=\"\" class=\"alignnone size-medium wp-image-1185\" width=\"300\" height=\"201\" srcset=\"https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/criblage-enz-2-300x201.png 300w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/criblage-enz-2-360x240.png 360w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/criblage-enz-2.png 395w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<div class=\"elementor-element elementor-element-61eed129 elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><span>Le <\/span><span>but de cette prestation est de mettre <\/span><span>au point et en <\/span><span>\u0153uvre une campagne de criblage pour l\u2019analyse de banques de <\/span><span>mutants. Plusieurs <\/span><span>approches existent qui permettent de s\u00e9lectionner parmi les banques de clones \u00e0 analyser, ceux qui pr\u00e9sentent les propri\u00e9t\u00e9s les plus int\u00e9ressantes :<\/span><\/p>\n<p><span>\u00a0 \u2022 tests <\/span><span>de s\u00e9lection<\/span><\/p>\n<p><span>\u00a0 \u2022 criblage <\/span><span>sur milieu solide<\/span><\/p>\n<p><span>\u00a0 \u2022 criblage <\/span><span>en milieu <\/span><span>liquide<\/span><\/p>\n<p><span>\u00a0 <\/span><span>\u2022 approche microplaque\/approche <\/span><span>microfluidique<\/span><span>. <\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-50205293 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-39f93019 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-453a6df5 elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-ICEO-Criblage-Enzymes.pdf\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-sm\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-a5346bc elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-1054e2ff elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"mailto:iceo@insa-toulouse.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>                    <\/div>\n\t\t        \n                    <div id=\"purification-denzymes-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"purification-denzymes-tab\">\n\t\t\t\t        <div class=\"elementor-element elementor-element-4b668149 elementor-widget elementor-widget-heading\">\n<div class=\"elementor-widget-container\">\n<h5 class=\"elementor-heading-title elementor-size-default\">Purification des Prot\u00e9ines \/ Enzymes<\/h5>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-110e6bf elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><span>La purification d\u2019une macromol\u00e9cule (prot\u00e9ine, enzyme, etc\u2026) est le pr\u00e9alable \u00e0 tous travaux visant la caract\u00e9risation fonctionnelle, structurale ou biophysique. Pour cela, PICT-ICEO dispose d\u2019un ensemble complet d\u2019\u00e9quipements constitu\u00e9 de chaines chromatographiques \u00c4KTA\u2122, des colonnes et des r\u00e9sines ainsi que d\u2019appareils accessoires (filtration tangentielle, etc\u2026) permettant de mettre en \u0153uvre une large gamme de techniques de s\u00e9paration. L\u2019automatisation des protocoles de purification multi-\u00e9tapes et multi-\u00e9chantillons est possible.<\/span><span><\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-448bb046 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-52690d63 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-3a83c8b3 elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/purif-enz1-300x251.png\" alt=\"\" class=\"alignnone size-medium wp-image-1188\" width=\"300\" height=\"251\" srcset=\"https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/purif-enz1-300x251.png 300w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/purif-enz1.png 543w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-bd4e33 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-332bba2d elementor-widget elementor-widget-image\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-image\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/purif-enz-2-220x300.png\" alt=\"\" class=\"alignnone size-medium wp-image-1189\" width=\"220\" height=\"300\" srcset=\"https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/purif-enz-2-220x300.png 220w, https:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2020\/01\/purif-enz-2.png 587w\" sizes=\"(max-width: 220px) 100vw, 220px\" \/><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<div class=\"elementor-element elementor-element-61eed129 elementor-widget elementor-widget-text-editor\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-text-editor elementor-clearfix\">\n<p><strong>Le plateau de purification de PICT-ICEO permet de :<\/strong><\/p>\n<p><span>\u2013 Mettre <\/span><span>au point des protocoles de purification sur mesure, permettant d\u2019atteindre un rendement et une puret\u00e9 satisfaisante<\/span><\/p>\n<p><span>\u2013 Mettre en \u0153uvre des protocoles d\u00e9j\u00e0 d\u00e9velopp\u00e9s pour la purification d\u2019une prot\u00e9ine.<\/span><\/p>\n<p><span>Deux modes d\u2019acc\u00e8s au plateau sont possibles: prestation et mise \u00e0 disposition des \u00e9quipements, avec ou sans assistance. <\/span><\/p>\n<p><strong><em>Compl\u00e9mentarit\u00e9 avec le plateau biophysique de PICT<\/em><\/strong><\/p>\n<p><span>Gr\u00e2ce \u00e0 la synergie avec le plateau biophysique de PICT, il est possible d\u2019effectuer la caract\u00e9risation biophysique de ces prot\u00e9ines\/enzymes (stabilit\u00e9, agr\u00e9gation, masse mol\u00e9culaire) ainsi que les \u00e9tudes structurales (RMN, cristallographie aux rayons X) et d\u2019interaction (DSF, ITC, RMN). <\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<section class=\"elementor-element elementor-element-50205293 elementor-section-boxed elementor-section-height-default elementor-section-height-default elementor-section elementor-inner-section\">\n<div class=\"elementor-container elementor-column-gap-default\">\n<div class=\"elementor-row\">\n<div class=\"elementor-element elementor-element-39f93019 elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-453a6df5 elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"http:\/\/www.pict.ipbs.fr\/wp-content\/uploads\/2021\/01\/Plateau-ICEO-Purification-de-ProtCines.pdf\" target=\"_blank\" class=\"elementor-button-link elementor-button elementor-size-sm\" rel=\"noopener\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"fa fa-file-pdf-o\"><\/i><\/span><span class=\"elementor-button-text\">en savoir plus<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"elementor-element elementor-element-a5346bc elementor-column elementor-col-50 elementor-inner-column\">\n<div class=\"elementor-column-wrap  elementor-element-populated\">\n<div class=\"elementor-widget-wrap\">\n<div class=\"elementor-element elementor-element-1054e2ff elementor-align-center elementor-widget elementor-widget-button\">\n<div class=\"elementor-widget-container\">\n<div class=\"elementor-button-wrapper\"><a href=\"mailto:iceo@insa-toulouse.fr\" class=\"elementor-button-link elementor-button elementor-size-sm\"><span class=\"elementor-button-content-wrapper\"><span class=\"elementor-button-icon elementor-align-icon-left\"><i class=\"far fa-address-book\"><\/i><\/span><span class=\"elementor-button-text\">contact<\/span><\/span><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/section>                    <\/div>\n\t\t                    <\/div>\n        <\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-28f7c5fc elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"28f7c5fc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Ing\u00e9nierie et d\u00e9couverte d\u2019enzymes originales Ing\u00e9nierie rationnelle et \u00e9volution dirig\u00e9e d\u2019enzymes; d\u00e9couverte d\u2019enzymes par m\u00e9tag\u00e9nomique fonctionnelle; criblage haut-d\u00e9bit d\u2019activit\u00e9s enzymatiques; production et purification d\u2019enzymes Le plateau PICT-ICEO est d\u00e9di\u00e9 au criblage et \u00e0 l\u2019\u00e9tude d\u2019activit\u00e9s enzymatiques originales, isol\u00e9es \u00e0 partir de librairies g\u00e9n\u00e9r\u00e9es par ing\u00e9nierie combinatoire des prot\u00e9ines ou issues [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":230,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/template-fullwidth.php","meta":{"footnotes":""},"class_list":["post-954","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/954","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/comments?post=954"}],"version-history":[{"count":49,"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/954\/revisions"}],"predecessor-version":[{"id":1905,"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/pages\/954\/revisions\/1905"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/media\/230"}],"wp:attachment":[{"href":"https:\/\/www.pict.ipbs.fr\/index.php\/wp-json\/wp\/v2\/media?parent=954"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}